Biosynthesis of penicillins; utilization of deuterophenylacetyl-N15-dl-valine in penicillin biosynthesis.
نویسندگان
چکیده
In Paper I in this series (2) the stimulation of penicillin production by certain compounds containing the phenylacetyl group was reported. It was realized that a stimulation of yield of this biological product following addition of a given substance to the medium may result from any one of several possible metabolic mechanisms. The stimulating substance may act as a vitamin or growth promoter, as a building block to be incorporated into the organism, or it may be required to satisfy any one of several other types of metabolic requirements. Thus, the early observations of increased yields were subject to varied interpretations, and there was need for proof of the direct utilization of the substances in penicillin biosynthesis. To provide such proof the preparation and use as a precursor of deuterophenylacetyl-N16-valine was proposed (L, IS, 17)-l Considerations in the choice of this particular compound included the knowledge that considerable specificity was exhibited by both acyl and amide portions of the molecule, and the fact that the amide portion of the molecule contained a carbon skeleton similar to that in penicillamine. The deuterophenylacetyl-N16valine was prepared in the Lilly laboratories (L, %3,8) from deuterophenyl-
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ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 175 2 شماره
صفحات -
تاریخ انتشار 1948